D8406

Sigma

 

Monoclonal Anti-DNP antibody produced in mouse

clone SPE-7, purified immunoglobulin, buffered aqueous solution

Synonym:Anti-Dinitrophenyl, Monoclonal Anti-DNP

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Description

Analysis NoteThe antibody has a minimum titer of 1:100,000 using a passive cutaneous anaphylaxis assay in rats. It is tested to ensure minimal reactivity against bovine serum albumin.
ImmunogenDNP-KLH conjugate
Physical formSolution in phosphate buffered saline, pH 7.4, containing 15 mM Sodium azide
ApplicationThe antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.

Properties

antibody formpurified immunoglobulin
cloneSPE-7 (monoclonal)
formbuffered aqueous solution
application(s)dot blot: suitable
 indirect ELISA: suitable
isotypeIgE
shipped indry ice
storage temp.−20°C

Safety

WGK Germany3

References

referenceEshhar, Z., et al., Generation of hybridomas secreting murine reaginic antibodies of anti-DNP specificity. J. Immunol. 124, 775, (1980)